Two cases of clump formation after thawing of hematopoietic progenitor cell products for transplantation

Cecilia Gamba; María del Rosario  Silvestri; Agustina Miguel; Danila Labonia; Nicolás Fernández Escobar; Silvia Mariana Pizzi; Daniel  Díaz Sánchez; Raquel Staciuk; Silvina Laura Kuperman

doi:10.46765/2675-374X.2025v6n1e257

Two cases of clump formation after thawing of hematopoietic progenitor cell products for transplantation

Authors

Cecilia Gamba Hospital de Pediatría Garrahan - HPC Processing Lab, Blood Bank – Buenos Aires, Argentina https://orcid.org/0009-0001-0091-801X
María del Rosario Silvestri Hospital de Pediatría Garrahan – HPC Processing Lab, Blood Bank – Buenos Aires, Argentina https://orcid.org/0009-0001-5138-955X
Agustina Miguel Hospital de Pediatría Garrahan – HPC Processing Lab, Blood Bank – Buenos Aires, Argentina https://orcid.org/0009-0006-1263-3480
Danila Labonia Hospital de Pediatría Garrahan – Bone Marrow Transplant Unit – Buenos Aires, Argentina https://orcid.org/0009-0003-9850-9576
Nicolás Fernández Escobar Hospital General de Niños Ricardo Gutierrez – Bone Marrow Transplant Unit – Buenos Aires, Argentina https://orcid.org/0009-0003-8325-8460
Silvia Mariana Pizzi Hospital de Pediatría Garrahan – Bone Marrow Transplant Unit – Buenos Aires, Argentina https://orcid.org/0009-0003-1418-4461
Daniel Díaz Sánchez Hospital de Pediatría Garrahan – Bone Marrow Transplant Unit – Buenos Aires, Argentina https://orcid.org/0009-0004-6452-4222
Raquel Staciuk Hospital de Pediatría Garrahan – Bone Marrow Transplant Unit – Buenos Aires, Argentina https://orcid.org/0009-0002-7770-2505
Silvina Laura Kuperman Hospital de Pediatría Garrahan – HPC Processing Lab, Blood Bank – Buenos Aires, Argentina https://orcid.org/0009-0000-6945-0091

DOI:

https://doi.org/10.46765/2675-374X.2025v6n1e257

Keywords:

Hematopoietic Progenitor Cells, Cryopreservation, Cell Aggregation

Abstract

While rare, clump formation has been described in the thawing of cryopreserved hematopoietic progenitor cell products intended for transplantation. When this occurs, it presents a significant challenge to the transplant team, potentially influencing the viability and safety of the product. Though the exact cause of clumping in thawed products remains unknown, studies have linked clump formation with the occurrence of adverse events. This report aimed to describe two cases of clump formation in thawed apheresis products observed in our processing laboratory, detailing the procedures followed and the outcomes achieved.

References

Cordoba R, Arrieta R, Kerguelen A, Hernandez-Navarro F. The occurrence of adverse events during the infusion of autologous peripheral blood stem cells is related to the number of granulocytes in the leukapheresis product. Bone Marrow Transplant. 2007;40(11):1063-7. https://doi.org/10.1038/sj.bmt.1705861

Siegenthaler MA, Vu DH, Ebnöther M, Ketterer N, Luthi F, Schmid P, Bargetzi M, Gasparini D, Tissot JD. ‘Agglutination and flocculation’ of stem cells collected by apheresis due to cryofibrinogen. Bone Marrow Transplant. 2004;33(7):765-7. https://doi.org/10.1038/sj.bmt.1704420

Mandanas RA, Kratochvil K, Garner T, Selby GB. Formation of fibrin clots in cryopreserved stem cell bags during thawing procedure: lack of impact on engraftment in autologous stem cell transplantation. Bone Marrow Transplant. 1999;23(3):303-4. https://doi.org/10.1038/sj.bmt.1701553

Reich-Slotky R, Patel N, Dael S, Semidei-Pomales M, Stephens H, Reich M, Schwartz J. Postthaw clotting of peripheral blood stem cell products due to insufficient anticoagulant. J Clin Apher. 2009;24(6):265-8. https://doi.org/10.1002/jca.20220

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Published

07/23/2025

How to Cite

Gamba, C., Silvestri , M. del R. ., Miguel, A., Labonia, D., Fernández Escobar, N., Pizzi , S. M., … Kuperman, S. L. (2025). Two cases of clump formation after thawing of hematopoietic progenitor cell products for transplantation. JOURNAL OF BONE MARROW TRANSPLANTATION AND CELLULAR THERAPY, 6(1). https://doi.org/10.46765/2675-374X.2025v6n1e257